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CORNELIA I. M. VAN DER VLUGT A. F. L. M. DERKS P. M. BOONEKAMP R. W. GOLDBACH 《The Annals of applied biology》1993,122(2):279-288
The influence of wounding and high-temperature treatment on the detection of iris severe mosaic virus (ISMV) in secondarily ISMV-infected iris bulbs was studied. Wounding of the bulbs just after lifting, followed by storage for 3 wk at 17°C or 20°C, increased the detectability of ISMV to 100% reliability. High-temperature treatment and consecutive storage at 17°C induced a similar improvement of detection. It is concluded that a certain degree of stress, such as wounding or high-temperature treatment, ultimately leads to an increase in viral antigens and thus to improvement of detection. It is hypothesised that the virus titre increases by the altered metabolism during the repair reactions as a response to stress applied to the bulbs. 相似文献
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W. J. VAN WAGTENDONK PHYLLIS H. GOLDMAN W. L. SMITH 《The Journal of eukaryotic microbiology》1970,17(3):389-391
SYNOPSIS Strains of the various syngens of Paramecium aurelia respond differently to the culture media developed for Strain 299 of syngen 8. Not only is there a variety of response between the syngens, but strains belonging to the same syngen respond differently to the 3 growth media. 相似文献
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SUMMARY Small stockwatering dams are usually ignored in the Transkei and Ciskei in fishfarming ventures. This paper reports on experimental results in fish production studies, using Aischgrund carp, which were obtained in the Ciskei and which indicate that economically worthwhile fish production can be achieved in such dams. 相似文献
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Summary After solubilization of membranes from vitellogenic oocytes of the crayfish Orconectes limosus, a component with an apparent molecular weight between 28 and 30 kDa that specifically binds vitellogenin, could be identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and by electroblotting. Because this component was pronase sensitive, we assume that we are dealing with a protein. A polyclonal rabbit antiserum was produced against this component and its tissue specificity was verified. 相似文献
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